Affiliation:
1. Institute of Biomedicine (IBIMED), Department of Medical Sciences (DCM) Universidade de Aveiro Portugal
2. Department of Chemistry, Mass Spectrometry Centre & LAQV‐REQUIMTE University of Aveiro Portugal
3. Department of Chemistry, CESAM—Centre for Environmental and Marine Studies University of Aveiro Portugal
Abstract
SETD7 (SET7/9, KMT7) is a lysine methyltransferase that targets master regulators of cell proliferation and differentiation. Here, the impact of inhibiting SETD7 catalytic activity on mammary epithelial cell differentiation was studied by focusing on genes associated with epithelial differentiation, lactogenesis, and lipid metabolism in HC11 and EpH4 cell lines. Setd7 mRNA and protein levels were induced upon lactogenic differentiation in both cell lines. Inhibition of SETD7 activity by the compound (R)‐PFI‐2 increased cell proliferation and downregulated E‐cadherin, beta‐catenin, lactoferrin, insulin‐like growth factor binding protein 5, and beta‐casein levels. In addition, inhibition of SETD7 activity affected the lipid profile and altered the mRNA expression of the phospholipid biosynthesis‐related genes choline phosphotransferase 1, and ethanolamine‐phosphate cytidylyltransferase. Altogether, the results suggest that inhibiting SETD7 catalytic activity impairs mammary epithelial and lactogenic differentiation.
Funder
Centro de Estudos Ambientais e Marinhos, Universidade de Aveiro
Fundação para a Ciência e a Tecnologia
Liga Portuguesa Contra o Cancro
Subject
Cell Biology,Genetics,Molecular Biology,Biochemistry,Structural Biology,Biophysics
Cited by
1 articles.
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