Affiliation:
1. State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine Peking Union Medical College Beijing China
2. Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine Peking Union Medical College Beijing China
Abstract
Endoplasmic reticulum (ER) is a highly complicated and dynamic organelle that actively changes its shape and communicates with other organelles. Visualization of ER in live cells is of great importance to understand cellular activities. Here, we designed a novel ER marker, RR‐mNeonGreen, which comprised an N‐terminal ER retention signal, a bright fluorescent protein (mNeonGreen), and a C‐terminal transmembrane region. Colocalization of RR‐mNeonGreen with mCherry‐KDEL verified that RR‐mNeonGreen perfectly labeled the ER. RR‐mNeonGreen showed better continuity of ER tubules when imaged by super‐resolution microscopy. Moreover, RR‐mNeonGreen is competent for live‐cell imaging of ER dynamics and tracing of the interaction between ER and mitochondria at high spatiotemporal resolution. In summary, RR‐mNeonGreen is a novel ER marker for super‐resolution live‐cell imaging with multiple merits.
Funder
National Key Research and Development Program of China
National Natural Science Foundation of China
Subject
Cell Biology,Genetics,Molecular Biology,Biochemistry,Structural Biology,Biophysics
Cited by
2 articles.
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