Affiliation:
1. Department of Biology, Iowa Neuroscience Institute University of Iowa Iowa City Iowa USA
2. Department of Pharmacology and Department of Ophthalmology, Visual, and Anatomical Sciences Wayne State University Detroit Michigan USA
3. The Jackson Laboratory Bar Harbor Maine USA
Abstract
AbstractThe Pcdhg gene cluster encodes 22 γ‐Protocadherin (γ‐Pcdh) cell adhesion molecules that critically regulate multiple aspects of neural development, including neuronal survival, dendritic and axonal arborization, and synapse formation and maturation. Each γ‐Pcdh isoform has unique protein domains—a homophilically interacting extracellular domain and a juxtamembrane cytoplasmic domain—as well as a C‐terminal cytoplasmic domain shared by all isoforms. The extent to which isoform‐specific versus shared domains regulate distinct γ‐Pcdh functions remains incompletely understood. Our previous in vitro studies identified protein kinase C (PKC) phosphorylation of a serine residue within a shared C‐terminal motif as a mechanism through which γ‐Pcdh promotion of dendrite arborization via myristoylated alanine‐rich C‐kinase substrate (MARCKS) is abrogated. Here, we used CRISPR/Cas9 genome editing to generate two new mouse lines expressing only non‐phosphorylatable γ‐Pcdhs, due either to a serine‐to‐alanine mutation (PcdhgS/A) or to a 15‐amino acid C‐terminal deletion resulting from insertion of an early stop codon (PcdhgCTD). Both lines are viable and fertile, and the density and maturation of dendritic spines remain unchanged in both PcdhgS/A and PcdhgCTD cortex. Dendrite arborization of cortical pyramidal neurons, however, is significantly increased in both lines, as are levels of active MARCKS. Intriguingly, despite having significantly reduced levels of γ‐Pcdh proteins, the PcdhgCTD mutation yields the strongest phenotype, with even heterozygous mutants exhibiting increased arborization. The present study confirms that phosphorylation of a shared C‐terminal motif is a key γ‐Pcdh negative regulation point and contributes to a converging understanding of γ‐Pcdh family function in which distinct roles are played by both individual isoforms and discrete protein domains.
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