Affiliation:
1. Leicester School of Pharmacy De Montfort University The Gateway Leicester LE1 9BH UK
2. School of Chemical Engineering University of Birmingham Edgbaston Birmingham B15 2TT UK
3. School of Chemistry University of Birmingham Edgbaston Birmingham B15 2TT UK
4. The Aptamer Group Windmill House Innovation Way Heslington York, YO10 5BR UK
Abstract
AbstractVirus recognition has been driven to the forefront of molecular recognition research due to the COVID‐19 pandemic. Development of highly sensitive recognition elements, both natural and synthetic is critical to facing such a global issue. However, as viruses mutate, it is possible for their recognition to wane through changes in the target substrate, which can lead to detection avoidance and increased false negatives. Likewise, the ability to detect specific variants is of great interest for clinical analysis of all viruses. Here, a hybrid aptamer‐molecularly imprinted polymer (aptaMIP), that maintains selective recognition for the spike protein template across various mutations, while improving performance over individual aptamer or MIP components (which themselves demonstrate excellent performance). The aptaMIP exhibits an equilibrium dissociation constant of 1.61 nM toward its template which matches or exceeds published examples of imprinting of the spike protein. The work here demonstrates that “fixing” the aptamer within a polymeric scaffold increases its capability to selectivity recognize its original target and points toward a methodology that will allow variant selective molecular recognition with exceptional affinity.
Funder
European Research Council
Engineering and Physical Sciences Research Council
Cited by
2 articles.
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