One-step purification, covalent immobilization, and additional stabilization of poly-His-tagged proteins using novel heterofunctional chelate-epoxy supports-Reference-Cited by-同舟云学术

One-step purification, covalent immobilization, and additional stabilization of poly-His-tagged proteins using novel heterofunctional chelate-epoxy supports

Published:2001-10-01 Issue:3 Volume:76 Page:269-276
ISSN:0006-3592
Container-title:Biotechnology and Bioengineering
language:en
Short-container-title:Biotechnol. Bioeng.

Author:

Mateo Cesar,Fernández-Lorente Gloria,Cortés Estrella,Garcia José L.,Fernández-Lafuente Roberto,Guisan José M.

Funder

European Community

Spanish CICYT

Resindion SRL (Mitsubishi Chemical Corp.)

Publisher

Wiley

Subject

Applied Microbiology and Biotechnology,Bioengineering,Biotechnology

Reference32 articles.

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3. A single step purification, immobilization and hyperactivation of lipases via interfacial adsorption on strongly hydrophobic supports;Bastida;Biotechnol Bioeng,1998

4. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding;Bradford;Anal Biochem,1976

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