Affiliation:
1. Department of Chemical Engineering University of Waterloo Waterloo Canada
2. Master of Technology Sanofi Toronto Canada
Abstract
AbstractA mathematical model is proposed for Bordetella pertussis with the main goal to better understand and describe the relation between cell growth, oxidative stress and NADPH levels under different oxidative conditions. The model is validated with flask experiments conducted under different conditions of oxidative stress induced by high initial glutamate concentrations, low initial inoculum and secondary culturing following exposure to starvation. The model exhibited good accuracy when calibrated and validated for the different experimental conditions. From comparisons of model predictions to data with different model mechanisms, it was concluded that intracellular reactive oxidative species only have an indirect effect on growth rate by reacting with NADPH and thereby reducing the amount of NADPH that is available for growth.
Reference67 articles.
1. Le microbe de la coqueluche;Bordet J;Ann I'Institut Pasteur,1906
2. ZavattiV.Monitoring of an Antigen Manufacturing Process Using Fluorescence(Master's thesis). University of Waterloo.2014.
3. Oxidative Stress Evokes a Metabolic Adaptation That Favors Increased NADPH Synthesis and Decreased NADH Production in
Pseudomonas fluorescens
4. ZavattiV.Application of Flow Cytometry and Fluorescence Spectroscopy to Monitor and Predict the Fermentation Activity in a Vaccine Manufacturing Process.2019.
5. Oxidative stress response ofInonotus obliquusinduced by hydrogen peroxide
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献