Affiliation:
1. Department of Ophthalmology The First Affiliated Hospital of Jinan University Guangzhou China
2. Key Laboratory for Regenerative Medicine, Ministry of Education, Department of Developmental and Regenerative Biology Jinan University Guangzhou China
3. Burn plastic surgery Longgang Central Hospital Shenzhen China
4. Department of Biomedical Engineering Jinan University Guangzhou China
5. Special Wards The First Affiliated Hospital of Jinan University Guangzhou China
Abstract
AbstractRecovery of skin function remains a significant clinical challenge for deep burns owing to the severe scar formation and poor appendage regeneration, and stem cell therapy has shown great potential for injured tissue regeneration. Here, a cell‐free therapy system for deep burn skin was explored using mesenchymal stem cell paracrine proteins (MSC‐PP) and polyethylene glycol (PEG) temperature‐sensitive hydrogels. A three‐dimensional (3D) dynamic culture system for MSCs' large‐scale expansion was established using a porous gelatin microcarrier crosslinked with hyaluronic acid (PGM‐HA), and the purified MSC‐PP from culture supernatant was characterized by mass spectrometric analysis. The results showed the 3D dynamic culture system regulated MSCs cell cycle, reduced apoptosis, and decreased lactic acid content, and the MSC‐PP produced in 3D group can promote cell proliferation, migration, and adhesion. The MSC‐PP + PEG system maintained stable release in 28 days of observation in vitro. The in vivo therapeutic efficacy was investigated in the rabbit's third‐degree burn model, and saline, PEG, MSC‐PP, and MSC‐PP + PEG treatments groups were set. The in vivo results showed that the MSC‐PP + PEG group significantly improved wound healing, inhibited scar formation, and facilitated skin appendage regeneration. In conclusion, the MSC‐PP + PEG sustained‐release system provides a potentially effective treatment for deep burn skin healing.
Funder
National Natural Science Foundation of China
Natural Science Foundation of Guangdong Province
Subject
Pharmaceutical Science,Biomedical Engineering,Biotechnology