Metabolomic‐Based Comparison of Daphnia magna and Japanese Medaka Responses After Exposure to Acetaminophen, Diclofenac, and Ibuprofen

Author:

Oliveira Pereira Erico A.1,Warriner Theresa R.2,Simmons Denina B. D.2ORCID,Jobst Karl J.3,Simpson André J.14,Simpson Myrna J.14ORCID

Affiliation:

1. Environmental Nuclear Magnetic Resonance Centre and Department of Physical and Environmental Sciences University of Toronto Scarborough Toronto Ontario Canada

2. Faculty of Science Ontario Tech University Oshawa Ontario Canada

3. Department of Chemistry Memorial University of Newfoundland St. John's Newfoundland and Labrador Canada

4. Department of Chemistry University of Toronto Toronto Ontario Canada

Abstract

AbstractPharmaceuticals are found in aquatic environments due to their widespread use and environmental persistence. To date, a range of impairments to aquatic organisms has been reported with exposure to pharmaceuticals; however, further comparisons of their impacts across different species on the molecular level are needed. In the present study, the crustacean Daphnia magna and the freshwater fish Japanese medaka, common model organisms in aquatic toxicity, were exposed for 48 h to the common analgesics acetaminophen (ACT), diclofenac (DCF), and ibuprofen (IBU) at sublethal concentrations. A targeted metabolomic‐based approach, using liquid chromatography–tandem mass spectrometry to quantify polar metabolites from individual daphnids and fish was used. Multivariate analyses and metabolite changes identified differences in the metabolite profile for D. magna and medaka, with more metabolic perturbations for D. magna. Pathway analyses uncovered disruptions to pathways associated with protein synthesis and amino acid metabolism with D. magna exposure to all three analgesics. In contrast, medaka exposure resulted in disrupted pathways with DCF only and not ACT and IBU. Overall, the observed perturbations in the biochemistry of both organisms were different and consistent with assessments using other endpoints reporting that D. magna is more sensitive to pollutants than medaka in short‐term studies. Our findings demonstrate that molecular‐level responses to analgesic exposure can reflect observations of other endpoints, such as immobilization and mortality. Thus, environmental metabolomics can be a valuable tool for selecting sentinel species for the biomonitoring of freshwater ecosystems while also uncovering mechanistic information. Environ Toxicol Chem 2024;43:1339–1351. © 2024 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

Funder

Natural Sciences and Engineering Research Council of Canada

Publisher

Wiley

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