Affiliation:
1. Postgraduate Program in Health and Aging Marilia Medical School (FAMEMA) Marilia São Paulo State Brazil
2. Programa de Pós‐Graduação em Ciências Farmacêuticas e Núcleo de Investigações Químico‐Farmacêuticas (NIQFAR) Vale do Itajaí University (UNIVALI) Itajaí Santa Catarina State Brazil
3. CECAV and Department of Genetics and Biotechnology University of Trás‐os‐Montes and Alto Douro Vila Real Portugal
4. Department of General Biology Londrina State University (UEL) Londrina Paraná State Brazil
5. Faculty of Philosophy and Sciences Speech and Hearing Therapy Department São Paulo State University (UNESP) Marília São Paulo State Brazil
Abstract
AbstractRubus imperialis Chum. Schl. (Rosaceae) have demonstrated some pharmacological activities, including gastroprotective action. However, genotoxic effects of R. imperialis extract was also reported. Since niga‐ichigoside F1 (NIF1) is a major compound of this plant species, and which has proven pharmacological properties, it is essential to investigate whether this compound is responsible for the observed toxicity. Therefore, the objective of this study was to analyze the effects of NIF1 on HepG2/C3A cells for possible cytogenotoxicity, cell cycle and apoptosis influence, and expression of genes linked to the DNA damage, cell cycle, cell death, and xenobiotic metabolism. The results showed no cytogenotoxic effects of NIF1 at concentrations between 0.1 and 20 μg/ml. Flow cytometry also showed no cell cycle or apoptosis disturbance. In the gene expression analysis, none of the seven genes investigated showed altered expression. The data indicate that NIF1 has no cytogenotoxic effects, and no interruption of the cell cycle, or induction of apoptosis, apparently not being responsible for the cytotoxic effects observed in the crude extract of R. imperialis.
Funder
Fundação de Amparo à Pesquisa do Estado de São Paulo
Conselho Nacional de Desenvolvimento Científico e Tecnológico
Cited by
2 articles.
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