Affiliation:
1. School of Pharmacy, Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica Nanjing University of Chinese Medicine Nanjing China
2. Department of Rheumatology Affiliated Hospital of Nanjing University of Chinese Medicine Nanjing China
3. The First Clinical Medical College Nanjing University of Chinese Medicine Nanjing China
Abstract
AbstractBackgroundRheumatoid arthritis (RA) is a persistent and systemic autoimmunity disease. The abnormal differentiation of Treg cells is important in pathogenesis. Despite previous studies showed that microRNAs (miRNAs, miR) are pivotal modulators of Treg cells, the effect of miRNAs on Treg cell differentiation and function is not clear. Our study wants to reveal the relationship of miR‐143‐3p with the differentiative ability and biofunction of Treg cells during the development of RA.MethodsThe Expressing level of miR‐143‐3p and cell factor generation in peripheral blood (PB) of RA sufferers were identified by ELISA or RT‐qPCR. The roles of miR‐143‐3p in Treg cell differentiation were studied via ShRNA/lentivirus transfection. Male DBA/1 J mice were separated into control, model, control mimics, and miR‐143‐3p mimics groups to analyze the anti‐arthritis efficacy, the differentiative ability of Treg cells, and the expression level of miR‐143‐3p.ResultsOur team discovered that the Expressing level of miR‐143‐3p was related to RA disease activities in a negative manner, and remarkably related to antiinflammation cell factor IL‐10. In vitro, the expression of miR‐143‐3p in the CD4+T cells upregulated the percentage of CD4+CD25+Fxop3+ cells (Tregs) and forkhead box protein 3 (Foxp3) mRNA expression. Evidently, miR‐143‐3p mimic intervention considerably upregulated the content of Treg cells in vivo, validly avoided CIA progression, and remarkably suppressed the inflammatory events of joints in mice.ConclusionOur findings indicated that miR‐143‐3p could ameliorate CIA through polarizing naive CD4+T cells into Treg cells, which may be a novel strategy to treat autoimmune diseases such as RA.
Funder
National Natural Science Foundation of China
Subject
Microbiology (medical),Biochemistry (medical),Medical Laboratory Technology,Clinical Biochemistry,Public Health, Environmental and Occupational Health,Hematology,Immunology and Allergy
Cited by
3 articles.
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