Explored potential hypoglycemic, hypolipidemic, and anti‐hyperuricemic components from Rheum tanguticum combing affinity ultrafiltration with four enzyme targets

Abstract

AbstractDue to its variety of biological activities, rhubarb has been widely applied worldwide with a long history. However, the potential bioactive compounds in Rheum tanguticum responsible for hypoglycemic, hypolipidemic, and anti‐hyperuricemic activities as well as their underlying mechanisms remain elusive. In order to explore these specific multifunctional ingredients, the in vitro α‐glucosidase and pancreatic lipase inhibitory assays were first determined, and the results indicated that R. tanguticum possessed remarkable hypoglycemic and hypolipidemic activities. Then, 14, 23, 15, and 10 active compounds in R. tanguticum were fast screened out by employing the bio‐affinity ultrafiltration with LC‐MS (Liquid Chromatograph‐Mass Spectrometer) strategy, which displayed various binding abilities to α‐glucosidase, α‐amylase, lipase, and xanthine oxidase enzymes, respectively. Further verifications of potential bioactive ingredients were implemented by molecule docking and the enzyme inhibitory assays in vitro. At last, the first interaction network between multifunctional components in R. tanguticum and their targets was further constructed facilitating to decipher the underlying mechanisms of action regarding R. tanguticum. The present work suggests that R. tanguticum may be a promising natural therapeutics and have good potential to be developed to prevent and treat diabetes, obesity, and gout in the near future.