Affiliation:
1. Departamento de Microbiologia Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais Belo Horizonte Minas Gerais Brazil
2. Fundação Ezequiel Dias, Diretoria do Instituto Octávio Magalhães, Serviço de Virologia e Riquetsioses Belo Horizonte Minas Gerais Brazil
Abstract
AbstractIn early May 2022, the first worldwide monkeypox virus (MPXV) outbreak was reported, with different clinical aspects from previously studied human monkeypox infections. Despite monkeypox medical importance, much of its biological aspects remain to be further investigated. In the present work, we evaluated ultrastructural aspects of MPXV asynchronous infections in Vero cells by transmission electron microscopy (TEM). The viral strain was isolated from a male patient infected during the 2022 outbreak. TEM analysis showed: (i) adhered intracellular mature virus particles before entry of the host cell; (ii) a reorganization of the rough endoplasmic reticulum cisternae into the so‐called “mini‐nuclei” structure associated with genome replication; and (iii) noticeably different sites within the viral factory presenting granular or fibrillar aspects. We also observed viral crescents, different MPXV particle morphotypes, and cellular alterations induced by infection, such as changes in the cytoskeleton structure and multimembrane vesicles abundance. Taken together, to the best of our knowledge, these results revealed for the first‐time ultrastructural aspects of different steps of the MPXV cycle.
Funder
Fundação de Amparo à Pesquisa do Estado de Minas Gerais
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Conselho Nacional de Desenvolvimento Científico e Tecnológico
Subject
Infectious Diseases,Virology
Reference33 articles.
1. A POX-LIKE DISEASE IN CYNOMOLGUS MONKEYS
2. A human infection caused by monkeypox virus in Basankusu Territory, Democratic Republic of the Congo;Ladnyj ID;Bull World Health Organ,1972
3. Isolation and properties of the causal agent of a new variola‐like disease (monkeypox) in man;Marennikova SS;Bull World Health Organ,1972
4. Centers for Disease Control and Prevention. (2022). Monkeypox. Past US cases and outbreaks.https://www.cdc.gov/poxvirus/monkeypox/outbreak/us-outbreaks.html
5. Two cases of monkeypox imported to the United Kingdom, September 2018
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