Membrane protein stability can be compromised by detergent interactions with the extramembranous soluble domains

Author:

Yang Zhengrong12,Wang Chi3,Zhou Qingxian2,An Jianli2,Hildebrandt Ellen4,Aleksandrov Luba A.56,Kappes John C.78,DeLucas Lawrence J.29,Riordan John R.56,Urbatsch Ina L.410,Hunt John F.3,Brouillette Christie G.12

Affiliation:

1. Department of Chemistry; University of Alabama at Birmingham; Birmingham Alabama

2. Center for Structural Biology; University of Alabama at Birmingham; Birmingham Alabama

3. Department of Biological Sciences; Columbia University; New York New York

4. Department of Cell Biology and Biochemistry; Texas Tech University Health Sciences Center; Lubbock Texas

5. Department of Biochemistry and Biophysics; The University of North Carolina at Chapel Hill; Chapel Hill North Carolina

6. Cystic Fibrosis Treatment and Research Center; The University of North Carolina at Chapel Hill; Chapel Hill North Carolina

7. Department of Medicine; University of Alabama at Birmingham; Birmingham Alabama

8. Birmingham Veterans Affairs Medical Center; Research Service; Birmingham Alabama

9. Department of Optometry; University of Alabama at Birmingham; Birmingham Alabama

10. Center for Membrane Protein Research; Texas Tech University Health Sciences Center; Lubbock TX

Funder

CFFT

NIH

Shared Facility Program of the UAB Comprehensive Cancer Center

Publisher

Wiley

Subject

Molecular Biology,Biochemistry

Reference102 articles.

1. A pedestrian guide to membrane protein crystallization;Wiener;Methods,2004

2. Membrane proteins, lipids and detergents: not just a soap opera;Seddon;Biochim Biophys Acta,2004

3. Detergents as tools in membrane biochemistry;Garavito;J Biol Chem,2001

4. Detergents for the stabilization and crystallization of membrane proteins;Prive;Methods,2007

5. Detergents: an overview;Linke;Methods Enzymol,2009

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