Affiliation:
1. Laboratory of Sensors Instrumentations and Processes (LCIP) Faculty of Science and Technology University of Abbes Laghrour 40000 Khenchela Algeria
2. Institute of Analytical Sciences (ISA) University of Lyon 5 Rue de La Doua 69100 Villeurbanne Lyon France
3. Center for Development of Advanced Technologies (CDTA), Baba Hassen 16081 Algiers Algeria
4. Laboratory of Properties and Interatomic Interactions (LASPI2A) Faculty of Science and Technology University of Abbes Laghrour 40000 Khenchela Algeria
5. Laboratory of Biology Water and Environment (LBEE) Faculty of SNV-STU. University of 8 May 1945 PO 401 24000 Guelma Algeria
Abstract
AbstractIn this study, a novel, sensitive electrochemical enzyme‐based biosensor for urea detection was presented. This biosensor combines a three‐electrode system consisting of a classic Glassy Carbon Electrode (GCE) as the working electrode, a platinum counter electrode, and Ag/AgCl as the reference electrode. To construct this urea platform, a GCE was modified with a polyaniline (PANi) film. Then, bacterial urease from Proteus mirabilis was immobilized on the modified GCE (Pm‐Urease‐PANi‐GCE). For the characterization of surface modification, Cyclic Voltammetry (CV) and Scanning Electron Microscope (SEM) were applied, while the Square Wave Voltammetry (SWV) technique was performed for urea detection. The main analytical characteristics of the Pm‐Urease‐PANi‐GCE biosensor showed a good linear range from 0.1 to 10 mM of urea, a limit of detection (LOD) of 0.1 mM, a Michaelis‐Menten Km of 0.23 mM, and a sensitivity value 46 μA/mM/cm2. This biosensor allows the detection of urea in solutions, and it could be improved for further medical, environmental, or engineering applications.
Subject
Electrochemistry,Analytical Chemistry
Cited by
4 articles.
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