TbEu(BTC) as a dNTP label for LAMP outcome in nucleic acid testing-Reference-Cited by-同舟云学术

TbEu(BTC) as a dNTP label for LAMP outcome in nucleic acid testing

Published:2023-03-22 Issue:6 Volume:35 Page:
ISSN:1040-0397
Container-title:Electroanalysis
language:en
Short-container-title:Electroanalysis

Author:

Gao Chenfeng¹,Wong Joseph J. Y.¹,Hall Elizabeth A. H.¹^ORCID

Affiliation:

1. Chemical Engineering and Biotechnology University of Cambridge Philippa Fawcett Drive Cambridge CB3 0AS

Abstract

AbstractLanthanide metal organic frameworks (MOFs), Eu(BTC) and TbEu(BTC) were assessed to follow uptake of deoxynucleotide triphosphate (dNTPs) in loop mediated isothermal amplification (LAMP) using BST2.0. dNTPs could be determined below 150 μM. A ratiometic measurement of Tb3+:Eu3+ fluorescence in TbEu(BTC) gave a limit of detection of 2.08 μM for dNTPs. dNTP uptake slowed above 8 mM Mg2+. This correlated with end‐point and real‐time LAMP, using PicoGreen and SYBR green respectively. For the P.Mal‐Lau 18S rRNA primer set, with P. malariae a detection limit of 102.5 copies was obtained. Cross reactivity between P. malariae and P. knowlesi was shown with 18S rRNA primer sets.

Publisher

Wiley

Subject

Electrochemistry,Analytical Chemistry

Link

https://onlinelibrary.wiley.com/doi/pdf/10.1002/elan.202200504

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5. Combining sequence-specific probes and DNA binding dyes in real-time PCR for specific nucleic acid quantification and melting curve analysis

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