Antibacterial and in vitro anticancer activities of the antimicrobial peptide NRC‐07 encapsulated in chitosan nanoparticles

Abstract

Antimicrobial peptides (AMPs) are promising alternatives to conventional antibiotics and chemotherapy in the treatment of multidrug‐resistant pathogens and drug‐resistant cancers. Clinical application of AMPs is limited due to low stability and inefficient transport. Encapsulation in nanocarriers may improve their therapeutic potential. Chitosan nanoparticles (CS‐NPs) are efficient carriers for proteins and peptides, improving the treatment of microbial infections and targeted drug delivery. We examined toxicity against cancer cell lines and antibacterial activities of the pleurocidin‐like AMP NRC‐07 upon encapsulation in CS‐NPs by ionotropic gelation. The biological activities of various formulations of free and encapsulated NRC‐07 and free nanoparticles were evaluated against Pseudomonas aeruginosa and breast cancer cells, using assays for cell viability and lactate dehydrogenase cytolysis with non‐cancer cell lines as controls. NRC‐07‐containing nanoparticles decreased the bacterial and cancer cell viability in a concentration‐dependent manner. Activities of encapsulated peptide were >2‐fold higher than those of free NRC‐07 peptide. Unloaded CS‐NPs and free peptide were not cytotoxic against control cells. Encapsulation of NRC‐07 into CS‐NPs enhanced the antibacterial and selective cytotoxicity of the peptide, possibly enhancing anticancer activities. Encapsulation presents a promising tool for the development of efficient drug delivery systems.