Combined Flow‐Fluorescence in situ hybridization to HHV‐8 and EBV reveals the viral heterogeneity of primary effusion lymphoma-Reference-Cited by-同舟云学术

Combined Flow‐Fluorescence in situ hybridization to HHV‐8 and EBV reveals the viral heterogeneity of primary effusion lymphoma

Published:2024-07-30 Issue:8 Volume:96 Page:
ISSN:0146-6615
Container-title:Journal of Medical Virology
language:en
Short-container-title:Journal of Medical Virology

Author:

Stammler Romain¹,Vacher Lauriane²,Fournier Benjamin³,Lemaire Pierre⁴,Chauvel Clémentine⁴,Silvestrini Marc‐Antoine¹³,Knapp Silène¹,de Frémont Grégoire Martin¹,Meignin Véronique⁵⁶,Salmona Maud⁷^ORCID,Legoff Jérôme⁷^ORCID,Vanjak Anthony¹^ORCID,Dunogué Bertrand⁸,Urbain Fanny⁹,Lambotte Olivier⁹,Noël Nicolas⁹,Gérard Laurence²⁵,Oksenhendler Eric²⁵,Galicier Lionel²⁵,Latour Sylvain³,Boutboul David¹³⁵¹⁰^ORCID

Affiliation:

1. Inserm U976 HIPI, Institut Universitaire d'Hématologie Université Paris Cité Paris France

2. Department of Clinical Immunology, Hôpital Saint Louis, AP‐HP Université Paris Cité Paris France

3. Laboratory of Lymphocyte Activation and Susceptibility to EBV infection, INSERM UMR 1163, Imagine Institute Université Paris Cité Paris France

4. Laboratory of Haematology, Hôpital Saint Louis, AP‐HP Université Paris Cité Paris France

5. National Reference Center for Castleman disease, Hôpital Saint Louis, AP‐HP Université Paris Cité Paris France

6. Pathology Department, Hôpital Saint Louis, AP‐HP Université Paris Cité Paris France

7. Laboratory of Virology, Hôpital Saint Louis, AP‐HP Université Paris Cité Paris France

8. Department of Internal Medicine, Hôpital Cochin, AP‐HP Université Paris Cité Paris France

9. Department of Internal Medicine, Hôpital Bicêtre, AP‐HP, Le Kremlin‐Bicêtre Université Paris Saclay Le Kremlin‐Bicetre France

10. Department of Hematology, Hôpital Cochin, AP‐HP Université Paris Cité Paris France

Abstract

AbstractPrimary effusion lymphoma (PEL) is a rare B‐cell non‐Hodgkin lymphoma associated with Kaposi Sarcoma‐associated herpesvirus (KSHV/HHV8) infection. Lymphoma cells are coinfected with Epstein‐Barr virus (EBV) in 60−80% of cases. Tools allowing a reliable PEL diagnosis are lacking. This study reports PEL diagnosis in 4 patients using a Flow‐Fluorescence in situ hybridization (FlowFISH) technique that allowed detection of differentially expressed EBV and HHV8 transcripts within the same sample, revealing viral heterogeneity of the disease. Moreover, infected cells exhibited variable expressions of CD19, CD38, CD40, and CD138. Therefore, FlowFISH is a promising tool to diagnose and characterize complex viral lymphoproliferations.

Publisher

Wiley

Link

https://onlinelibrary.wiley.com/doi/pdf/10.1002/jmv.29836

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