Molecular mapping of QTL for type I Fusarium head blight resistance in two recombinant inbred lines wheat populations

Abstract

AbstractFusarium head blight (FHB) is a major concern for wheat production and food safety, globally. Resistance to infection (type I resistance) is important for breeding varieties with good FHB resistance. To better understand the genetic architecture underlying type I FHB resistance, quantitative trait loci (QTL) mapping was conducted using two recombinant inbred line populations derived from the crosses Yangmai 4 (YM4)/Yanzhan 1 (YZ1) and Yangmai 5 (YM5)/YZ1. A total of five resistance QTL, including QFhb.yaas‐2D, QFhb.yaas‐4D, QFhb.yaas‐5B, QFhb.yaas‐6B, and QFhb.yaas‐3A, were detected from these two populations. Except for QFhb.yaas‐2D and QFhb.yaas‐5B, the resistance effects of all the other QTLs are derived from YM4 or YM5. QFhb.yaas‐4D is a common QTL detected both in YM4/YZ1 and YM5/YZ1 populations. QFhb.yaas‐2D, QFhb.yaas‐4D, and QFhb.yaas‐5B were colocalized with known plant height (PH) genes Rht8, Rht‐D1, and flowering date (FD) gene Vrn‐B1. However, QFhb.yaas‐6B and QFhb.yaas‐3A were not associated with PH and FD. Kompetitive Allele‐Specific PCR markers for QFhb.yaas‐6B and QFhb.yaas‐3A had been developed and validated in an additional panel of 211 wheat cultivars/lines. The result showed that these two resistance alleles decreased 46.65% of the percentage of diseased spikelets. These results provide valuable information for the fine mapping of QFhb.yaas‐6B and QFhb.yaas‐3A in the future.