Affiliation:
1. School of Biomedical Engineering (Suzhou), Division of Life Sciences and Medicine University of Science and Technology of China Hefei China
2. Jiangsu Key Laboratory of Medical Optics Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Science Suzhou China
Abstract
AbstractWe present a method to use a birefringent crystal for generating two illumination beams in a digital scanned laser light‐sheet microscopy (DSLM) system. Upon this, a conventional confocal DSLM can be easily upgraded to a dual‐slit confocal DSLM with two‐fold imaging speed. We have implemented this method to our bidirectional DSLM system, locating two identical calcite crystals on both illumination paths from both sides of the sample. The neurons of in vivo larval zebrafish have been fast imaged with sterling image quality, especially ~2.5 times higher contrast, compared to the conventional DSLM.
Subject
General Physics and Astronomy,General Engineering,General Biochemistry, Genetics and Molecular Biology,General Materials Science,General Chemistry