Affiliation:
1. Bio‐photonics and Green‐photonics Laboratory, Department of Physics Indian Institute of Technology Delhi New Delhi India
2. Department of Oral Pathology and Microbiology Centre for Dental Education & Research, All India Institute of Medical Sciences (AIIMS) Delhi India
Abstract
AbstractMany optical techniques have been used in various diagnostics and biomedical applications since a decade and polarization imaging is one of the non‐invasive and label free optical technique to investigate biological samples making it an important tool in diagnostics, biomedical applications. We report a multispectral polarization‐based imaging of oral tissue by utilizing a polarization microscope system with a broadband‐light source. Experiments were performed on oral tissue samples and multispectral Stokes mapping was done by recording a set of intensity images. Polarization‐based parameters like degree of polarization, angle of fast axis, retardation and linear birefringence have been retrieved. The statistical moments of these polarization components have also been reported at multiples wavelengths. The polarimetric properties of oral tissue at different stages of cancer have been analyzed and significant changes from normal to pre‐cancerous lesions to the cancerous are observed in linear birefringence quantification as (1.7 ± 0.1) × 10−3, (2.5 ± 0.2) × 10−3 and (3.3 ± 0.2) × 10−3 respectively.
Subject
General Physics and Astronomy,General Engineering,General Biochemistry, Genetics and Molecular Biology,General Materials Science,General Chemistry