Affiliation:
1. Department of Electrical Engineering and Computer Science Florida Atlantic University Boca Raton Florida USA
2. Center for Molecular Biology and Biotechnology Florida Atlantic University Boca Raton Florida USA
Abstract
AbstractMetformin hydrochloride, an antihyperglycemic agent, and sulindac, a nonsteroidal anti‐inflammatory drug, are FDA‐approved drugs known to exert anticancer effects. Previous studies demonstrated sulindac and metformin's anticancer properties through mitochondrial dysfunction and inhibition of mitochondrial electron transport chain complex I and key signaling pathways. In this study, various drugs were administered to A549 lung cancer cells, and results revealed that a combination of sulindac and metformin enhanced cell death compared to the administration of the drugs separately. To measure superoxide production over time, we employed a time‐lapse fluorescence imaging technique using mitochondrial‐targeted hydroethidine. Fluorescence microscopy data showed the most significant increases in superoxide production in the combination treatment of metformin and sulindac. Results showed significant differences between the combined drug treatment and control groups and between the positive control and control groups. This approach can be utilized to quantify the anticancer efficacy of drugs, creating possibilities for additional therapeutic options.
Funder
Florida Atlantic University
National Institutes of Health
Subject
General Physics and Astronomy,General Engineering,General Biochemistry, Genetics and Molecular Biology,General Materials Science,General Chemistry
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
1. In-Vehicle Sensing and Data Analysis for Older Drivers with Mild Cognitive Impairment;2023 IEEE 20th International Conference on Smart Communities: Improving Quality of Life using AI, Robotics and IoT (HONET);2023-12-04