Affiliation:
1. Science Medical Center Saratov State University Saratov Russia
2. A.V. Zelmann Center for Neurobiology and Brain Rehabilitation Skolkovo Institute of Science and Technology Moscow Russia
3. School of Engineering and Materials Science Queen Mary University of London London UK
Abstract
AbstractFluorescent photoconvertible materials and molecules have been successfully exploited as bioimaging markers and cell trackers. Recently, the novel fluorescent photoconvertible polymer markers have been developed that allow the long‐term tracking of individual labeled cells. However, it is still necessary to study the functionality of this type of fluorescent labels for various operating conditions, in particular for commonly used discrete wavelength lasers. In this article, the photoconversion of fluorescent polymer labels with both pulsed and continuous‐wave lasers with 532 nm‐irradiation wavelength, and under different laser power densities were studied. The photoconversion process was described and its possible mechanism was proposed. The peculiarities of fluorescent polymer capsules performance as an aqueous suspension and as a single capsule were described. We performed the successful nondestructivity marker photoconversion inside RAW 264.7 monocyte/macrophage cells under continuous‐wave laser with 532 nm‐irradiation wavelength, showing prospects of these fluorescent markers for long‐term live cell labeling.
Funder
Russian Science Foundation
Subject
General Physics and Astronomy,General Engineering,General Biochemistry, Genetics and Molecular Biology,General Materials Science,General Chemistry
Cited by
1 articles.
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