Affiliation:
1. Life Science Technologies Department Imec Leuven Belgium
2. Laboratory for Integrated Nanodiagnostics Johns Hopkins University School of Medicine Baltimore Maryland USA
3. Department of Pathology Johns Hopkins University School of Medicine Baltimore Maryland USA
Abstract
AbstractWe demonstrate a portable, compact system to perform absorption‐based enzymatic assays at a visible wavelength of 639 nm on a photonic waveguide‐based sensor chip, suitable for lab‐on‐a‐chip applications. The photonic design and fabrication of the sensor are described, and a detailed overview of the portable measurement system is presented. In this publication, we use an integrated photonic waveguide‐based absorbance sensor to run a full enzymatic assay. An assay to detect creatinine in plasma is simultaneously performed on both the photonic sensor on the portable setup and on a commercial microplate reader for a clinically relevant creatinine concentration range. We observed a high correlation between the measured waveguide propagation loss and the optical density measurement from the plate reader and measured a limit‐of‐detection of 4.5 μM creatinine in the sensor well, covering the relevant clinical range for creatinine detection.
Subject
General Physics and Astronomy,General Engineering,General Biochemistry, Genetics and Molecular Biology,General Materials Science,General Chemistry