Optimized E. coli expression strain LOBSTR eliminates common contaminants from His‐tag purification

Author:

Andersen Kasper R.1,Leksa Nina C.1,Schwartz Thomas U.1

Affiliation:

1. Department of BiologyMassachusetts Institute of Technology77 Massachusetts AvenueCambridge Massachusetts02139

Funder

Lundbeck Foundation and a Sapere Aude DFF postdoc grant

NIH, Pew Scholar Award

Publisher

Wiley

Subject

Molecular Biology,Biochemistry,Structural Biology

Reference15 articles.

1. Genetic Approach to Facilitate Purification of Recombinant Proteins with a Novel Metal Chelate Adsorbent

2. An Escherichia coli protein consisting of a domain homologous to FK506‐binding proteins (FKBP) and a new metal binding motif;Wülfing C;J Biol Chem,1994

3. Structural analysis and classification of native proteins from E. coli commonly co-purified by immobilised metal affinity chromatography

4. Structure and Function of Both Domains of ArnA, a Dual Function Decarboxylase and a Formyltransferase, Involved in 4-Amino-4-deoxy-L-arabinose Biosynthesis

5. slyD, a host gene required for phi X174 lysis, is related to the FK506‐binding protein family of peptidyl‐prolyl cis‐trans‐isomerases;Roof WD;J Biol Chem,1994

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