Sequential immunofluorescence staining and image analysis for detection of large numbers of antigens in individual cell nuclei

Author:

Wählby Carolina,Erlandsson Fredrik,Bengtsson Ewert,Zetterberg Anders

Funder

Swedish Foundation for Strategic Research (SFF)

Swedish Cancer Society and Cancerföreningen in Stockholm

Svenska Läkaresällskapet

Karolinska Institute

Publisher

Wiley

Subject

Cell Biology,Endocrinology,Hematology,Biophysics,Pathology and Forensic Medicine

Reference23 articles.

1. Immunological properties of an antibody containing a fluorescent group;Coons;Proc Soc Exp Biol Med,1941

2. The demonstration of pneumococcal antigen in tissues by the use of fluorescent antibody;Coons;J Immunol,1942

3. Contrasting fluorescent labels for two antibodies;Silverstein;J Histochem Cytochem,1957

4. The use of a vertical illuminator with interchangeable dichroic mirrors for fluorescence microscopy with incident light;Ploem;Z Wissensch Mikr,1967

5. An indirect immunofluorescence procedure for staining the same cryosection with two mouse monoclonal primary antibodies;Lewis;J Histochem Cytochem,1993

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