Immuno‐Desorption Electrospray Ionization Mass Spectrometry Imaging Identifies Functional Macromolecules by Using Microdroplet‐Cleavable Mass Tags

Author:

Song Xiaowei12,Zang Qingce3,Li Chao4,Zhou Tianhao5,Zare Richard N.1ORCID

Affiliation:

1. Department of Chemistry Stanford University Stanford CA-94305 USA

2. Department of Chemistry Fudan University Shanghai 200016 China

3. Institution of Materia Medica Chinese Academy of Medical Science and Peking Union Medical College Beijing China

4. Department of Medicine Stanford University School of Medicine Palo Alto CA-94304 USA

5. National Clinical Research Center of Cancer Tianjin Medical University Tianjin 300060 China

Abstract

AbstractWe present immunoassay‐based desorption electrospray ionization mass spectrometry imaging (immuno‐DESI‐MSI) to visualize functional macromolecules such as drug targets and cascade signaling factors. A set of boronic acid mass tags (BMTs) were synthesized to label antibodies as MSI probes. The boronic ester bond is employed to cross‐link the BMT with the galactosamine‐modified antibody. The BMT can be released from its tethered antibody by ultrafast cleavage of the boronic ester bond caused by the acidic condition of sprayed DESI microdroplets containing water. The fluorescent moiety enables the BMT to work in both optical and MS imaging modes. The positively charged quaternary ammonium group enhances the ionization efficiency. The introduction of the boron element also makes mass tags readily identified because of its unique isotope pattern. Immuno‐DESI‐MSI provides an appealing strategy to spatially map macromolecules beyond what can be observed by conventional DESI‐MSI, provided antibodies are available to the targeted molecules of interest.

Funder

Air Force Office of Scientific Research

National Natural Science Foundation of China

Publisher

Wiley

Subject

General Medicine

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