Affiliation:
1. Institute of Organic Chemistry University of Freiburg Alberstrasse 21 79104 Freiburg i. Br. Germany
2. Institute of Pharmaceutical Science University of Freiburg Sonnenstrasse 5 79104 Freiburg i. Br. Germany
3. Institute of Inorganic and Analytical Chemistry University of Freiburg Alberstrasse 21 79104 Freiburg i. Br. Germany
Abstract
AbstractHerein, we describe the creation of an artificial protein cage housing a dual‐metal‐tagged guest protein that catalyzes a linear, two‐step sequential cascade reaction. The guest protein consists of a fusion protein of HaloTag and monomeric rhizavidin. Inside the protein capsid, we established a ruthenium‐catalyzed allylcarbamate deprotection reaction followed by a gold‐catalyzed ring‐closing hydroamination reaction that led to indoles and phenanthridines with an overall yield of up to 66 % in aqueous solutions. Furthermore, we show that the encapsulation stabilizes the metal catalysts against deactivation by air, proteins and cell lysate.
Funder
Deutsche Forschungsgemeinschaft