Enzyme‐Polymer‐Conjugate‐Based Pickering Emulsions for Cell‐Free Expression and Cascade Biotransformation

Author:

Lu Haofan1,Ouyang Jingping2,Liu Wan‐Qiu1,Wu Changzhu23ORCID,Li Jian1ORCID

Affiliation:

1. School of Physical Science and Technology ShanghaiTech University Shanghai 201210 China

2. Department of Physics Chemistry and Pharmacy University of Southern Denmark Campusvej 55 5230 Odense Denmark

3. Danish Institute for Advanced Study (DIAS) University of Southern Denmark Campusvej 55 5230 Odense Denmark

Abstract

AbstractIn this study, we addressed the limitations of conventional enzyme‐polymer‐conjugate‐based Pickering emulsions for interfacial biocatalysis, which traditionally suffer from nonspecific and uncontrollable conjugation positions that can impede catalytic performance. By introducing a non‐canonical amino acid (ncAA) at a specific site on target enzymes, we enabled precise polymer‐enzyme conjugation. These engineered conjugates then acted as biocatalytically active emulsifiers to stabilize Pickering emulsions, while encapsulating a cell‐free protein synthesis (CFPS) system in the aqueous phase for targeted enzyme expression. The resulting cascade reaction system leveraged enzymes expressed in the aqueous phase and on the emulsion interface for optimized chemical biosynthesis. The use of the cell‐free system eliminated the need for intact whole cells or purified enzymes, representing a significant advancement in biocatalysis. Remarkably, the integration of Pickering emulsion, precise enzyme‐polymer conjugation, and CFPS resulted in a fivefold enhancement in catalytic performance as compared to traditional single‐phase reactions. Therefore, our approach harnesses the combined strengths of advanced biochemical engineering techniques, offering an efficient and practical solution for the synthesis of value‐added chemicals in various biocatalysis and biotransformation applications.

Funder

National Natural Science Foundation of China

Danmarks Frie Forskningsfond

Novo Nordisk Fonden

Carlsbergfondet

Publisher

Wiley

Subject

General Medicine

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3