Spatiotemporal Control over Polynucleotide Brush Growth on DNA Origami Nanostructures

Author:

Yang Yunqi1,Lu Qinyi2,Chen Yu3,DeLuca Marcello4,Arya Gaurav1,Ke Yonggang5,Zauscher Stefan1ORCID

Affiliation:

1. Department of Mechanical Engineering and Materials Science Department of Biomedical Engineering Duke University Durham NC 27708 USA

2. Department of Chemistry Emory University Atlanta GA 30322 USA

3. University Program in Materials Science and Engineering Duke University Durham NC 27708 USA

4. Department of Mechanical Engineering and Materials Science Duke University Durham NC 27708 USA

5. Department of Biomedical Engineering Georgia Institute of Technology and Emory University Atlanta GA 30322 USA

Abstract

AbstractDNA nanotechnology provides an approach to create precise, tunable, and biocompatible nanostructures for biomedical applications. However, the stability of these structures is severely compromised in biological milieu due to their fast degradation by nucleases. Recently, we showed how enzymatic polymerization could be harnessed to grow polynucleotide brushes of tunable length and location on the surface of DNA origami nanostructures, which greatly enhances their nuclease stability. Here, we report on strategies that allow for both spatial and temporal control over polymerization through activatable initiation, cleavage, and regeneration of polynucleotide brushes using restriction enzymes. The ability to site‐specifically decorate DNA origami nanostructures with polynucleotide brushes in a spatiotemporally controlled way provides access to “smart” functionalized DNA architectures with potential applications in drug delivery and supramolecular assembly.

Funder

National Institute of Biomedical Imaging and Bioengineering

Basic Energy Sciences

National Science Foundation

Publisher

Wiley

Subject

General Medicine

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