Affiliation:
1. College of Engineering and Applied Sciences Nanjing National Laboratory of Microstructures Jiangsu Key Laboratory of Artificial Functional Materials Nanjing University Nanjing Jiangsu 210023 China
2. College of Chemistry and Molecular Sciences Wuhan University Wuhan Hubei 430072 China
3. State Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical Engineering Chemistry and Biomedicine Innovation Center (ChemBIC) Nanjing University Nanjing Jiangsu 210023 China
Abstract
AbstractNanomaterials with enzyme‐like activities, termed as nanozymes, have found wide applications in various fields. It has been a long‐term aim to rationally design and synthesize highly active nanozymes and thus to further improve their application performance. Guided by the nanoconfinement effect, we confine cytochrome c (Cyt c) within a mesoporous metal–organic framework (MOF), PCN‐222 nanoparticle (NP), forming a protein/MOF hybrid nanozyme, termed as Cyt c@PCN‐222 NP. The confined Cyt c exhibits around 3–4‐fold higher peroxidase‐like activity than free Cyt c. Due to the increase in the activity of Cyt c, the Cyt c@PCN‐222 NPs exhibit a quite low limit of detection (≈0.13 μM) towards H2O2. Sonication‐induced H2O2 formation in water by using a lab‐quipped ultrasonic cleaner can be sensitively probed, which suggests that H2O2‐sensitive materials should be carefully handled during the utilization of ultrasonic equipment. We speculate that this nanoconfinement strategy can broaden our synthetic methodology for the rational design of nanozymes.
Funder
National Natural Science Foundation of China
National Key Research and Development Program of China
Priority Academic Program Development of Jiangsu Higher Education Institutions
Fundamental Research Funds for the Central Universities