Affiliation:
1. Clinical Laboratory Shanghai Children's Medical Center School of Medicine Shanghai Jiao Tong University Shanghai 200127 P. R. China
2. Shanghai Key Laboratory of Clinical Molecular Diagnostics for Pediatrics Shanghai 200127 P. R. China
3. Department of Clinical Laboratory Medicine Shanghai Tenth People's Hospital of Tongji University Shanghai 200072 P. R. China
4. Sanya Women and Children's Hospital Managed by Shanghai Children's Medical Center Sanya 572000 P. R. China
Abstract
AbstractFunctional imaging (FI) techniques have revolutionized tumor imaging by providing information on specific tumor functions, such as glycometabolism. However, tumor cells lack unique molecular characteristics at the molecular level and metabolic pathways, resulting in limited metabolic differences compared to normal cells and increased background signals from FI. To address this limitation, we developed a novel imaging technique termed proximity‐enhanced functional imaging (PEFI) for accurate visualization of tumors. By using “two adjacent chemically labeled glycoproteins” as output signals, we significantly enhance the metabolic differences between tumor and normal cells by PEFI, thereby reducing the background signals for analysis and improving the accuracy of tumor functional imaging. Our results demonstrate that PEFI can accurately identify tumors at the cellular, tissue, and animal level, and has potential value in clinical identification and analysis of tumor cells and tissues, as well as in the guidance of clinical tumor resection surgery.
Funder
Natural Science Foundation of Shanghai Municipality
National Natural Science Foundation of China