Varying the Stiffness and Diffusivity of Rod‐Shaped Microgels Independently through Their Molecular Building Blocks

Abstract

AbstractMicrogels are water‐swollen, crosslinked polymers that are widely used as colloidal building blocks in scaffold materials for tissue engineering and regenerative medicine. Microgels can be controlled in their stiffness, degree of swelling, and mesh size depending on their polymer architecture, crosslink density, and fabrication method—all of which influence their function and interaction with the environment. Currently, there is a lack of understanding of how the polymer composition influences the internal structure of soft microgels and how this morphology affects specific biomedical applications. In this report, we systematically vary the architecture and molar mass of polyethylene glycol‐acrylate (PEG‐Ac) precursors, as well as their concentration and combination, to gain insight in the different parameters that affect the internal structure of rod‐shaped microgels. We characterize the mechanical properties and diffusivity, as well as the conversion of acrylate groups during photopolymerization, in both bulk hydrogels and microgels produced from the PEG‐Ac precursors. Furthermore, we investigate cell‐microgel interaction, and we observe improved cell spreading on microgels with more accessible RGD peptide and with a stiffness in a range of 20 kPa to 50 kPa lead to better cell growth.