Affiliation:
1. Institute of Molecular Medicine Department of Breast Surgery Renji Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200127 China
2. MOE Key Laboratory of Spectrochemical Analysis and Instrumentation Collaborative Innovation Center of Chemistry for Energy Materials State Key Laboratory of Physical Chemistry of Solid Surfaces Key Laboratory for Chemical Biology of Fujian Province Department of Chemical Biology College of Chemistry and Chemical Engineering Xiamen University Xiamen 361005 China
Abstract
AbstractThe protein phenotypes of extracellular vesicles (EVs) have emerged as promising biomarkers for cancer diagnosis and treatment monitoring. However, the technical challenges in rapid isolation and multiplexed molecular detection of EVs have limited their clinical practice. Herein, we developed a magnetically driven tandem chip to achieve streamlined rapid isolation and multiplexed profiling of surface protein biomarkers of EVs. Driven by magnetic force, the magnetic nanomixers not only act as tiny stir bars to promote mass transfer and enhance reaction efficiency of EVs, but also transport on communicating vessels of the tandem chip continuously and expedite the assay workflow. We designed cyclic surface enhancement of Raman scattering (SERS) tags to bind with target EVs and then release them by exonuclease I, eliminating steric hindrance and amplifying the SERS signal of multiple protein biomarkers on EVs. Due to the excellent assay performance, six breast cancer biomarkers were detected simultaneously on EVs using only 10 μL plasma within 1.5 h. The unweighted SUM signature offers great accuracy in discriminating breast cancer patients from healthy donors. Overall, the dynamic magnetic driving tandem chip offers a new avenue to advance the clinical application of EV‐based liquid biopsy.
Funder
Key Technologies Research and Development Program
National Natural Science Foundation of China
Fundamental Research Funds for the Central Universities