A method for cleaning of used disposable plastic parts from automated RNA extraction system

Author:

Kumar Awanish1,Shamni Ofer2,Peretz Ayelet3,Magenheim Judith3,Klochendler Agnes3,Fridlich Ori3,Neiman Daniel3,Oiknine‐Djian Esther4,Vorontsov Olesya4,Sido Tal5,Wolf Dana G.4,Dor Yuval3,Domb Abraham J.1ORCID

Affiliation:

1. School of Pharmacy‐Faculty of Medicine, The Hebrew University of Jerusalem Institute of Drug Research Jerusalem Israel

2. Cyclotron/Radiochemistry Unit Hadassah/Hebrew University Hospital Jerusalem Israel

3. Department of Developmental Biology and Cancer Research The Institute for Medical Research Israel‐Canada, The Hebrew University‐Hadassah Medical School Jerusalem Israel

4. The Lautenberg center for immunology and cancer research, The Institute for Medical Research Israel‐Canada, Faculty of Medicine Hebrew University of Jerusalem Jerusalem Israel

5. Mathematics Department Bar‐Ilan University Ramat‐Gan Israel

Abstract

AbstractDuring the first wave of COVID‐19 worldwide pandemic, the number of testing in Israel was severely affected by the shortage of reagents and consumables required to operate the instrument performing the sample extraction before the reverse transcription‐polymerase chain reaction (RT‐PCR), namely QIAsymphony instrument (Qiagen, Hilden, Germany). We aimed to develop an assay to clean used plastic ware parts while maintaining the reliability and reproducibility of the RT‐PCR testing. Wells, rods cover, and tips used by the QIAsymphony instrument were washed using distilled water, 3% sodium hypochlorite or sodium hydroxide 0.01 M solutions, followed by an additional wash using distilled water and eventually using 3% hydrogen peroxide or ethanol solution. Following overnight drying, the cleaned parts were installed in the QIAsymphony instrument, and the reliability of the results was compared to the set of samples that were analyzed using a new plastic ware. The results of reusing the cleaned sets of consumables were comparable to those of the new sets.

Publisher

Wiley

Subject

Polymers and Plastics

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