Elucidating the structural and functional prophecy of the Rv2326c gene, an ABC transporter of Mycobacterium tuberculosis H37Rv through computational approach
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Published:2023-08-22
Issue:6
Volume:70
Page:2025-2037
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ISSN:0885-4513
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Container-title:Biotechnology and Applied Biochemistry
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language:en
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Short-container-title:Biotech and App Biochem
Author:
Sharma Priyanka1,
Kumar Ajit12,
Meena Laxman S.12ORCID
Affiliation:
1. CSIR—Institute of Genomics and Integrative Biology Delhi India
2. Academy of Scientific and Innovative Research (AcSIR) CSIR‐HRDC Ghaziabad Uttar Pradesh India
Abstract
AbstractTuberculosis is a fatal disease caused by Mycobacterium tuberculosis. M. tuberculosis becoming drug‐resistant day by day, necessitating to know the mechanism behind the drug resistance and how to overcome this deadly malady. Drug resistance and reduced drug bioavailability are caused by a class of transporter proteins called the ATP‐binding cassette (ABC) transporters, which pump a range of medicines out of cells at the price of ATP hydrolysis. By using computational approaches, we tried to elaborate the probable function of the Rv2326c gene of M. tuberculosis, perhaps involved in drug resistance mechanism. The presence of the signature motif of ABC transporters (LSGGELQRLALAAAL and LSGGQMRRVVLAGLL) and ATP binding motif (GXXXXGKT and GXXXXGKS) in the protein sequence signifying its importance in the ATP binding and transportation of molecules. Further, this manuscript elaborated about tertiary structure and validation, functional category, localization, phosphorylation site prediction, mutational analysis of conserved motifs. Ligand docking study shows the highest affinity with ATP than GTP justified its function as an ATP binding protein. The Rv2326c protein is present in the inner membrane and working as an ATP binding protein and might be playing a dynamic role in transportation. In this study, we found that Rv2326c protein might be working as an ABC transporter by which the drugs and other molecules are imported or exported into the bacterium. As a result, the current study provides a means to better understand its normal functioning and basic biology, which can help in the development of novel therapeutic targeting approaches for Rv2326c protein.
Funder
Council of Scientific and Industrial Research, India
Department of Science and Technology, Ministry of Science and Technology, India
Subject
Process Chemistry and Technology,Drug Discovery,Applied Microbiology and Biotechnology,Biomedical Engineering,Molecular Medicine,General Medicine,Bioengineering,Biotechnology