Glycine‐replaced epinecidin‐1 variant bestows better stability and stronger antimicrobial activity against a range of nosocomial pathogenic bacteria

Author:

Jeyarajan Sivakumar123ORCID,Peter Ansu Susan1,Ranjith Sukumar1,Sathyan Aswathy1,Duraisamy Senbagam14,Kandasamy Indira1,Chidambaram Prahalathan5,Kumarasamy Anbarasu1

Affiliation:

1. Microbial Biotechnology Laboratory, Department of Marine Biotechnology Bharathidasan University Tiruchirappalli Tamil Nadu India

2. Department of Pharmacology University of Michigan Ann Arbor Michigan USA

3. Transgeinc Animal Model Core, Biomedical Research Core Facilities University of Michigan Ann Arbor Michigan USA

4. Department of Biotechnology, Faculty of Science and Humanities SRM Institute of Science and Technology Kattankulathur Tamil Nadu India

5. Department of Biochemistry Bharathidasan University Tiruchirappalli Tamil Nadu India

Abstract

AbstractEpinecidin‐1 (epi‐1), an antimicrobial peptide first identified in marine grouper fish, has multifunctional bioactivities. The present study aims to improve its therapeutic potential via structural modifications that could enhance its antimicrobial activity and stability. To achieve it, we replaced glycine and the first histidine in the parent epi‐1 with lysine, which resulted in a peptide with a repeating KXXK motif and improved physiochemical properties related to antimicrobial activity. This modified peptide, referred to as glycine‐to‐lysine replaced‐epi‐1, also gained stability and a twofold increase in helical propensity. To produce the active peptide, overlap extension PCR was employed to generate the gene of GK‐epi‐1 via site‐directed mutagenesis, which was then cloned into the pET‐32a vector and expressed as a recombinant fusion protein in Escherichia coli C43 (DE3) strain. The recombinant protein was purified and digested with enterokinase to release the active peptide fragment, which was then evaluated for antimicrobial activity and stability. The lysine substitution led to an enhancement in broad‐spectrum antimicrobial activity against a wide range of nosocomial pathogenic bacteria.

Publisher

Wiley

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3