Single‐Cell Glycan and RNA Sequencing (scGR‐seq)

Abstract

AbstractSingle‐cell multi‐omics analysis has emerged as a crucial tool in modern biology due to its capacity to uncover the intricate molecular heterogeneity within individual cells. Glycan structural diversity on the cell surface creates a "cell signature" that varies by cell type and state. However, single‐cell glycan analysis remains a challenge. This protocol presents a series of techniques and procedures that enable the simultaneous measurements of glycan and RNA at the single‐cell level via DNA‐barcoded lectin‐based sequencing (scGR‐seq). The techniques include (1) the preparation of DNA‐barcoded lectins, (2) a step‐by‐step protocol for single‐cell glycan/RNA sequencing, and (3) a data analysis approach for the integration of glycan and RNA data. © 2023 Wiley Periodicals LLC.Basic Protocol 1: Preparation of DNA‐barcoded lectinsBasic Protocol 2: Single‐cell glycan/RNA sequencing