Affiliation:
1. School of Biomedical Engineering Shanghai Jiao Tong University Shanghai 200030 China
2. Department of Chemistry Institute of Molecular Aggregation Science Tianjin University Tianjin 300072 China
3. Biomedical Engineering Department and International Cancer Institute Peking University Beijing 100081 China
Abstract
AbstractTwo‐photon photodynamic therapy (TP‐PDT), which utilizes near‐infrared light to excite photosensitizer via two‐photon excitation (TPE), is a promising modality in the treatment of deeply seated tumors or thick tumors. However, the TPE domain is much smaller than the tumor volume, significantly limiting the therapeutical outcomes of TP‐PDT. Here, a light‐sheet TPE system is designed and constructed using a cylindrical lens for highly efficient TP‐PDT. The light‐sheet TPE performance is characterized and optimized via theoretical analysis and experimental studies in solution, phantom, and tumor. The optimized excitation system can reach a large TPE domain of 2.6 × 2.7 × 0.09 mm in the tumor, which is not achievable using conventional TPE methods, enabling TPE and subsequently generated reactive oxygen species to cover the whole tumor under line‐scanning. Outstanding TP‐PDT therapeutic performance of 70% tumor growth inhibition rate is achieved under line‐scanned light‐sheet TPE, making the proposed light‐sheet excited TP‐PDT a potential therapeutic tool for future translational research.
Funder
Ministry of Science and Technology of the People's Republic of China
National Natural Science Foundation of China
Shanghai Jiao Tong University