Poly‐ε‐caprolactone (PCL)/poly‐l‐lactic acid (PLLA) nanofibers loaded by nanoparticles‐containing TGF‐β1 with linearly arranged transforming structure as a scaffold in cartilage tissue engineering

Author:

Kalvand Elham123,Bakhshandeh Haleh24,Nadri Samad56,Habibizadeh Mina7,Rostamizadeh Kobra58

Affiliation:

1. Department of Pharmaceutical Biomaterials, School of Pharmacy Zanjan University of Medical Sciences Zanjan Iran

2. Department of Nanobiotechnology Pasteur Institute of Tehran Tehran Iran

3. Department of Nanotechnology and Tissue Engineering Stem Cell Technology Research of Tehran Tehran Iran

4. New Technologies Research Group, Department of Nanobiotechnology Pasteur Institute of Iran Tehran Iran

5. Zanjan Pharmaceutical Nanotechnology Research Center Zanjan University of Medical Sciences Zanjan Iran

6. Department of Medical Nanotechnology, School of Medicine Zanjan University of Medical Sciences Zanjan Iran

7. Regenerative Medicine Research Center Kermanshah University of Medical Sciences Kermanshah Iran

8. Pharmaceutical Biomaterials Department, School of Pharmacy Zanjan University of Medical Sciences Zanjan Iran

Abstract

AbstractThis study aimed to present a novel three‐dimensional nanocomposite scaffold using poly‐ε‐caprolactone (PCL), containing transforming growth factor‐beta 1 (TGF‐β1)‐loaded chitosan‐dextran nanoparticles and poly‐l‐lactic acid (PLLA), to make use of nanofibers and nanoparticles simultaneously. The electrospinning method fabricated a bead‐free semi‐aligned nanofiber composed of PLLA, PCL, and chitosan‐dextran nanoparticles containing TGF‐β1. A biomimetic scaffold was constructed with the desired mechanical properties, high hydrophilicity, and high porosity. Transmission electron microscopy findings showed a linear arrangement of nanoparticles along the core of fibers. Based on the results, burst release was not observed. The maximum release was achieved within 4 days, and sustained release was up to 21 days. The qRT‐PCR results indicated an increase in the expression of aggrecan and collagen type Ι genes compared to the tissue culture polystyrene group. The results indicated the importance of topography and the sustained release of TGF‐β1 from bifunctional scaffolds in directing the stem cell fate in cartilage tissue engineering.

Funder

Zanjan University of Medical Sciences

Publisher

Wiley

Subject

Metals and Alloys,Biomedical Engineering,Biomaterials,Ceramics and Composites

Cited by 2 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3