Affiliation:
1. Department of Biomedical Engineering Columbia University New York New York USA
2. Department of Biological Sciences Columbia University New York New York USA
Abstract
AbstractInduced Tregs (iTregs) have great promise in adoptive immunotherapy for treatment of autoimmune diseases. This report investigates the impacts of substrate stiffness on human Treg induction, providing a powerful yet simple approach to improving production of these cells. Conventional CD4+ human T cells were activated on materials of different elastic modulus and cultured under suppressive conditions. Enhanced Treg induction was observed on softer materials as early as 3 days following activation and persisted for multiple weeks. Substrate stiffness also affected epigenetic modification of Treg specific genes and Treg suppressive capacity. Tregs induced on substrates of an optimal stiffness balance quantity and suppressive quality.
Funder
National Institutes of Health
National Science Foundation Graduate Research Fellowship Program
National Center for Advancing Translational Sciences
Cited by
1 articles.
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