Positive effect of acellular amniotic membrane dressing with immobilized growth factors in skin wound healing

Author:

Laleh Mahsa12,Tahernejad Mahrokh12,Bonakdar Shahin1,Asefnejad Azadeh3ORCID,Golkar Majid4,Kazemi‐Lomedasht Fatemeh5,Habibi‐Anbouhi Mahdi1

Affiliation:

1. National Cell Bank of Iran Pasteur Institute of Iran Tehran Iran

2. Faculty of Medical Sciences and Technologies, Science and Research Branch Islamic Azad University Tehran Iran

3. Department of Biomedical Engineering, Science and Research Branch Islamic Azad University Tehran Iran

4. Molecular Parasitology Laboratory, Parasitology Department Pasteur Institute of Iran Tehran Iran

5. Biotechnology Research Center, Venom and Biotherapeutics Molecules Lab Pasteur Institute of Iran Tehran Iran

Abstract

AbstractThe human amniotic membrane dressing has been shown to accelerate the wound healing process in the clinic. In this study, heparin was conjugated to a human Acellular Amniotic Membrane (hAAM) to provide affinity binding sites for immobilizing growth factors. To study the acceleration of the wound healing process, we bound epidermal growth factor and fibroblast growth factor 1 to heparinized hAAMs (GF‐Hep‐hAAMs). The heparinized hAAMs (Hep‐hAAMs) were characterized by toluidine blue staining and infrared spectroscopy. The quality control of hAAM was performed by hematoxylin staining, swelling capacity test and biomechanical evaluation. The cytotoxicity, adhesion, and migration in vitro assays of GF‐Hep‐hAAMs on L‐929 fibroblast cells were also studied by MTT assay, scanning electron microscopy, and scratch assay, respectively. Finally, in vivo skin wound healing study was performed to investigate the wound closure rate, re‐epithelization, collagen deposition, and formation of new blood vessels. The results showed that GF‐Hep‐hAAMs enhance the rate of wound closure and epidermal regeneration in BALB/c mice. In conclusion, GF‐Hep‐hAAMs could accelerate the wound healing process, significantly in the first week.

Funder

Pasteur Institute of Iran

Publisher

Wiley

Subject

Metals and Alloys,Biomedical Engineering,Biomaterials,Ceramics and Composites

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