Hierarchization of Myogenic and Adipogenic Progenitors Within Human Skeletal Muscle

Author:

Pisani Didier F.1,Clement Noémie12,Loubat Agnès3,Plaisant Magali1,Sacconi Sabrina12,Kurzenne Jean-Yves4,Desnuelle Claude12,Dani Christian1,Dechesne Claude A.1

Affiliation:

1. Institute of Developmental Biology and Cancer, University of Nice Sophia-Antipolis, CNRS, UMR6543, Nice, France

2. Centre de Référence Maladies Neuromusculaires et de la SLA, CHU de Nice, Nice, France

3. Plateforme de Cytométrie, IFR50, Faculté de Médecine, University of Nice Sophia-Antipolis, Nice, France

4. Services de Chirurgie Pédiatrique, CHU de Nice, Hôpital l'Archet, Nice, France

Abstract

Abstract Skeletal muscle cells constitute a heterogeneous population that maintains muscle integrity through a high myogenic regenerative capacity. More unexpectedly, this population is also endowed with an adipogenic potential, even in humans, and intramuscular adipocytes have been found to be present in several disorders. We tested the distribution of myogenic and adipogenic commitments in human muscle-derived cells to decipher the cellular basis of the myoadipogenic balance. Clonal analysis showed that adipogenic progenitors can be separated from myogenic progenitors and, interestingly, from myoadipogenic bipotent progenitors. These progenitors were isolated in the CD34+ population on the basis of the expression of CD56 and CD15 cell surface markers. In vivo, these different cell types have been found in the interstitial compartment of human muscle. In vitro, we show that the proliferation of bipotent myoadipogenic CD56+CD15+ progenitors gives rise to myogenic CD56+CD15− progenitors and adipogenic CD56−CD15+ progenitors. A cellular hierarchy of muscle and fat progenitors thus occurs within human muscle. These results provide cellular bases for adipogenic differentiation in human skeletal muscle, which may explain the fat development encountered in different muscle pathological situations.

Funder

Centre National de la Recherche Scientifique

Publisher

Oxford University Press (OUP)

Subject

Cell Biology,Developmental Biology,Molecular Medicine

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