Combination MEG3 lncRNA and Ciprofloxacin dramatically decreases cell migration and viability as well as induces apoptosis in GC cells in vitro

Author:

Najafi Dena1,Siri Goli2,Sadri Maryam3,Yazdani Omid4,Esbati Romina5,Karimi Parvin6,Keshavarz Ali7,Mehmandar‐Oskuie Amirreza8,Ilktac Mehmet1ORCID

Affiliation:

1. Faculty of Pharmacy Eastern Mediterranean University Famagusta North Cyprus Turkey

2. Department of Internal Medicine Amir Alam Hospital, Tehran University of Medical Sciences Tehran Iran

3. Department of Internal Medicine Tabriz University of Medical Sciences Tabriz Iran

4. Department of Medical Science, School of Medicine Shahid Beheshti University Tehran Iran

5. Research Center for Social Determinants of Health, Research Institute for Endocrine Sciences Shahid Beheshti University of Medical Sciences Tehran Iran

6. Fars Population‐Based Cancer Registry Shiraz University of Medical Sciences Shiraz Iran

7. Department of Hematology and Blood Banking, School of Allied Medical Sciences Shahid Beheshti University of Medical Sciences Tehran Iran

8. Department of Immunology, School of Public Health Tehran University of Medical Sciences Tehran Iran

Abstract

AbstractGastric cancer (GC) is a prominent cause of cancer‐related mortality worldwide. Long noncoding RNA (lncRNA) maternal expression gene3 (MEG3) participates in numerous signaling pathways by targeting the miRNA‐mRNA axis. Studies on human tumors have demonstrated that the antibiotic Ciprofloxacin induces cell cycle changes, programmed cell death, and growth suppression. In this study, we transfected MEG3 lncRNA and Ciprofloxacin into the MKN‐45 GC cell line. qRT‐PCR was employed to evaluate the effects on the specific microRNA and mRNA. The wound healing test, MTT assay, and flow cytometry were used to assess the impact of their administration on cell migration, viability, and apoptosis, respectively. Research showed that miR‐147 expression fell even more after MEG3 lncRNA transfection, leading to an increase in B‐cell lymphoma 2 (BCL‐2) levels. Ciprofloxacin transfection did not significantly affect the axis, except for MEG3, which led to its slight upregulation. MEG3 lncRNA inhibited the migration of MKN‐45 cells compared to the control group. When MEG3 lncRNA was coupled with Ciprofloxacin, there was a significant reduction in cell migration compared to untreated groups and controls. MTT assay and flow cytometry demonstrated that MEG3 lncRNA decreased cell viability and triggered apoptosis. Simultaneous administration of MEG3 lncRNA and Ciprofloxacin revealed a significant reduction in cell viability caused by increased apoptosis obtained from MTT or flow cytometry assays. Modulating the miR‐147‐BCL‐2 axis decreases cell migration and survival while promoting cell death. In conclusion, combining MEG3 lncRNA with Ciprofloxacin may be an effective therapeutic approach for GC treatment by influencing the miR‐14‐BCl‐2 axis, resulting in reduced cell viability, migration, and increased apoptosis.

Publisher

Wiley

Reference38 articles.

1. Updates on global epidemiology, risk and prognostic factors of gastric cancer

2. MicroRNAs as non-invasive diagnostic biomarkers for gastric cancer: Current insights and future perspectives

3. Chemotherapy for advanced gastric cancer;Wagner AD;Cochrane Database Syst Rev,2017

4. Genomic and epigenomic biomarkers in colorectal cancer: From diagnosis to therapy

5. Gastric cancer: classification, histology and application of molecular pathology;Hu B;J Gastrointest Oncol,2012

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3