Mutational scan inferred binding energetics and structure in intrinsically disordered protein CcdA

Author:

Chandra Soumyanetra1,Manjunath Kavyashree2,Asok Aparna1,Varadarajan Raghavan1ORCID

Affiliation:

1. Molecular Biophysics Unit, Indian Institute of Science Bangalore India

2. Institute for Stem Cell Science and Regenerative Medicine Bangalore India

Abstract

AbstractUnlike globular proteins, mutational effects on the function of Intrinsically Disordered Proteins (IDPs) are not well‐studied. Deep Mutational Scanning of a yeast surface displayed mutant library yields insights into sequence‐function relationships in the CcdA IDP. The approach enables facile prediction of interface residues and local structural signatures of the bound conformation. In contrast to previous titration‐based approaches which use a number of ligand concentrations, we show that use of a single rationally chosen ligand concentration can provide quantitative estimates of relative binding constants for large numbers of protein variants. This is because the extended interface of IDP ensures that energetic effects of point mutations are spread over a much smaller range than for globular proteins. Our data also provides insights into the much‐debated role of helicity and disorder in partner binding of IDPs. Based on this exhaustive mutational sensitivity dataset, a rudimentary model was developed in an attempt to predict mutational effects on binding affinity of IDPs that form alpha‐helical structures upon binding.

Funder

Science and Engineering Research Board

Publisher

Wiley

Subject

Molecular Biology,Biochemistry

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