Identification of 2‐Methylthio‐methylenethio‐N6‐(cis‐4‐hydroxyisopentenyl)adenosine (msms2io6A37) as a Novel Modification at Adenosine 37 of tRNAs from Salmonella typhimurium

Abstract

AbstractPost‐transcriptional modifications of tRNA nucleotide are important determinants in folding, structure and function. We have successfully identified and characterized a new modified base named 2‐methylthio‐methylenethio‐N6‐(cis‐4‐hydroxyisopentenyl)adenosine, which is present at position 37 in some tRNAs. We also showed that this new modified adenosine is derived from the known 2‐methylthio‐methylenethio‐N6‐(isopentenyl)adenosine nucleoside by a catalytic cycle of the tRNA‐diiron monooxygenase, MiaE, present in Salmonella typhimurium.