Affiliation:
1. School of Chemistry Food Biosciences and Pharmacy University of Reading Whiteknights Reading RG6 6AD UK
2. DUBBLE-CRG @ ESRF CS40220 The European Synchrotron 71, Avenue des Martyrs 38043 Grenoble Cedex 9 France
Abstract
AbstractThe influence of alpha‐cyclodextrin (αCD) on PEG crystallization is examined for a peptide‐PEG conjugate, YYKLVFF‐PEG3k comprising an amyloid peptide YYKLVFF linked to PEG with molar mass 3 kg mol−1. Remarkably, differential scanning calorimetry (DSC) and simultaneous synchrotron small‐angle/wide‐angle X‐ray scattering (SAXS/WAXS) show that crystallization of PEG is suppressed by αCD, provided that the cyclodextrin content is sufficient. A hexagonal mesophase is formed instead. The αCD threading reduces the conformational flexibility of PEG, and hence suppresses crystallization. These results show that addition of cyclodextrins can be used to tune the crystallization of peptide‐polymer conjugates and potentially other polymer/biomolecular hybrids.
Funder
Engineering and Physical Sciences Research Council