Affiliation:
1. Cell Biology and Biophysics Unit European Molecular Biology Laboratory Meyerhofstraße 1 69117 Heidelberg Germany
Abstract
AbstractVisualizing the structure and dynamics of biomolecules is critical to understand biological function, and requires methods to fluorescently label targets of interest in their cellular context. Self‐labelling proteins, which combine a genetically encoded tag with a small‐molecule fluorophore, have attracted considerable attention for this purpose, as they can overcome limitations of fluorescent proteins. Among them, the HaloTag protein is the most broadly used, showing fast specific labelling with a small, easy to functionalize and cell‐permeant ligand. Synthetic chemistry and protein engineering have provided a portfolio of powerful imaging tools exploiting HaloTag, along with general methods to optimize and adapt them to specific applications. Here, we provide an overview of fluorescent reporters based on the HaloTag protein for imaging and biosensing, highlighting engineering strategies and general applications.
Funder
European Molecular Biology Laboratory
Subject
Organic Chemistry,Molecular Biology,Molecular Medicine,Biochemistry
Reference155 articles.
1. Fluorescent Proteins and Their Applications in Imaging Living Cells and Tissues
2. Specific Covalent Labeling of Recombinant Protein Molecules Inside Live Cells
3. Note: Both the terms “chemigenetic” and “chemogenetic” have been used to refer to such protein-dye labelling systems. The term “chemogenetic” is however strongly associated with methods of chemical manipulation of cellular processes particularly of neuronal function. For this reason we prefer to use the term “chemigenetic” in the context of self-labelling protein-based imaging tools.
4.
5. A general method for the covalent labeling of fusion proteins with small molecules in vivo
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