A Bifunctional Methyltransferase in Biosynthesis of Antitumor Antibiotic Streptonigrin-Reference-Cited by-同舟云学术

A Bifunctional Methyltransferase in Biosynthesis of Antitumor Antibiotic Streptonigrin

Published:2024-08-22 Issue: Volume: Page:
ISSN:1439-4227
Container-title:ChemBioChem
language:en
Short-container-title:ChemBioChem

Author:

Wang Xiaozheng¹,Xie Xinyue¹,Wo Jing¹²,Huang Tingting¹³,Deng Zixin¹³,Lin Shuangjun¹³⁴

Affiliation:

1. State Key Laboratory of Microbial Metabolism Joint International Laboratory of Metabolic & Developmental Sciences School of Life Sciences & Biotechnology Shanghai Jiao Tong University 200240 Shanghai China

2. Institute of Engineering Biology and Health Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals College of Pharmaceutical Sciences Zhejiang University of Technology 310014 Hangzhou, Zhejiang China

3. Haihe Laboratory of Synthetic Biology 300308 Tianjin China

4. Frontiers Science Center for Transformative Molecules Shanghai Jiao Tong University 200240 Shanghai China

Abstract

AbstractStreptonigrin (STN, 1) is a highly functionalized aminoquinone alkaloid antibiotic with broad and potent antitumor activity. STN structurally contains four methyl groups belonging to two types: C‐methyl group and O‐methyl groups. Here, we report the biochemical characterization of the O‐methyltransferase StnQ2 that can catalyze both the methylation of a hydroxyl group and a carboxyl group in the biosynthesis of streptonigrin. This work not only provides a new insight into methyltransferases, but also advances the elucidation of the complete biosynthetic pathway of streptonigrin.

Funder

National Key Research and Development Program of China

National Natural Science Foundation of China

Program of Shanghai Subject Chief Scientist

Publisher

Wiley

Reference31 articles.