Measuring Ligand‐bound Protein Complexes with Proximity Labeling: A Practical Guide-Reference-Cited by-同舟云学术

Measuring Ligand‐bound Protein Complexes with Proximity Labeling: A Practical Guide

Published:2024-04-16 Issue:10 Volume:25 Page:
ISSN:1439-4227
Container-title:ChemBioChem
language:en
Short-container-title:ChemBioChem

Author:

Goyal Pavitra¹^ORCID,Tao Andrew J.¹^ORCID,Mumby Elizabeth J.²^ORCID,English Justin G.²^ORCID,Ferguson Fleur M.¹³^ORCID

Affiliation:

1. Department of Chemistry and Biochemistry University of California San Diego La Jolla CA 92093

2. Department of Biochemistry University of Utah School of Medicine Salt Lake City UT 84112

3. Skaggs School of Pharmacy and Pharmaceutical Sciences University of California San Diego La Jolla CA 92093

Abstract

AbstractIdentifying the drug‐target interactome of small molecule therapeutics is essential for understanding the full pharmacological effects of a compound. These therapies often induce changes within the cellular proteome, leading to unexpected consequences such as changes in the targets complexation state or off‐target interactions between the compound and additional proteins. Currently, unbiased target‐ID approaches are being used to embark on this task. Here we provide an overview of the strengths and limitations of these methods, and a practical step‐by‐step protocol for using the BioTAC system to assist with drug target and interactome ID.

Funder

National Institutes of Health

University of California, San Diego

Publisher

Wiley

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