Construction of DNA Bilayer Tiles and Arrays Using Circular DNA Molecules as Scaffolds

Abstract

AbstractUsing oligonucleotides to weave 2D tiles such as double crossovers (DX) and multi‐arm junction (mAJ) tiles and arrays is well‐known, but weaving 3D tiles is rare. Here, we report the construction of two new bilayer tiles in high yield using small circular 84mer oligonucleotides as scaffolds. Further, we designed five E‐tiling approaches to construct porous nanotubes of microns long in medium yield via solution assembly and densely covered planar microscale arrays via surface‐mediated assembly.